Myh10^{tm6(Myh9/GFP)Rsad}
Targeted Allele Detail

Summary

• Symbol: Myh10^{tm6(Myh9/GFP)Rsad}
• Name: myosin, heavy polypeptide 10, non-muscle; targeted mutation 6, Robert S Adelstein
• MGI ID: MGI:3775953
• Synonyms: B^a*, GFP-NMHC II-A
• Gene: Myh10 Location: Chr11:68582385-68707458 bp, + strand Genetic Position: Chr11, 41.95 cM, cytoband B3
• Alliance: Myh10^{tm6(Myh9/GFP)Rsad} page

Cardiac defects during embryonic development in Myh10^{tm6(Myh9/GFP)Rsad}/Myh10^{tm6(Myh9/GFP)Rsad} mice

Show the 5 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:124610
• Parent Cell Line: CMT-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: cDNA encoding a GFP-tagged nonmuscle myosin IIA heavy chain was inserted into exon 2 of the nonmuscle myosin IIB (Myh10) locus, placing nonmuscle myosin IIA under control of the IIB promoter, expressing IIA in place of IIB, and ablating IIB. (J:124610)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Myh10 Mutation: 95 strains or lines available

References

• Original: J:124610 Bao J, et al., Replacement of nonmuscle myosin II-B with II-A rescues brain but not cardiac defects in mice. J Biol Chem. 2007 Jul 27;282(30):22102-11
• All: 5 reference(s)