Wwox^Gt(XG218)Byg
Gene trapped Allele Detail

Summary

• Symbol: Wwox^Gt(XG218)Byg
• Name: WW domain-containing oxidoreductase; gene trap XG218, BayGenomics
• MGI ID: MGI:3775954
• Synonyms: Wwox^gt
• Gene: Wwox Location: Chr8:115166395-116079447 bp, + strand Genetic Position: Chr8, 60.99 cM, cytoband E1
• Alliance: Wwox^Gt(XG218)Byg page

Mutation origin

• Mutant Cell Line: XG218
• Germline Transmission: Earliest citation of germline transmission: J:132078
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: A gene trap vector containing a beta-geo cassette was inserted into intron 4 and resulted in a fusion protein at exon 4. Real-time PCR shows that mRNA levels are reduced by 95% in embryos and 85-95% in adults, depending on tissue examined. Protein is undetectable in most adult tissues, however, low levels are detected in testes. This is a hypomorph. (J:132078)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XG218	CC200173 ; (MGI Seq Detail)	5' RACE	Chr8:115166400-115175075 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  22 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wwox Mutation: 59 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:132078 Ludes-Meyers JH, et al., WWOX hypomorphic mice display a higher incidence of B-cell lymphomas and develop testicular atrophy. Genes Chromosomes Cancer. 2007 Dec;46(12):1129-36
• All: 2 reference(s)