Lepr^tm2(cre)Rck
Targeted Allele Detail

Summary

• Symbol: Lepr^tm2(cre)Rck
• Name: leptin receptor; targeted mutation 2, Jeffrey M Friedman
• MGI ID: MGI:3776158
• Synonyms: LeprB-Cre, LepRb^Cre, LepRb-IRES-Cre, Lepr^cre, ObRb-Cre, Obrb-IRES-Cre
• Gene: Lepr Location: Chr4:101574601-101672549 bp, + strand Genetic Position: Chr4, 46.96 cM
• Alliance: Lepr^tm2(cre)Rck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68414
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Lepr^tm2(cre)Rck expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Lepr (MGI:104993)

• Mutation details: A cre recombinase expression cassette containing an IRES domain and a FRT-flanked neomcyin cassette was placed immediately downstream of the stop codon for the gene. Flp-mediated recombination removed the FRT-flanked neo cassette. Cre expression in the hypothalamus was confirmed by use of a virus containing a cre-dependent reporter gene. (J:68414)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Lepr (mouse) Summary of all recombinase alleles driven by Lepr. Comparative matrix view of recombinase activities Alliance mouse Lepr gene page

Expression

In Mice Carrying this Mutation:

18 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lepr Mutation: 122 strains or lines available

References

• Original: J:68414 DeFalco J, et al., Virus-assisted mapping of neural inputs to a feeding center in the hypothalamus. Science. 2001 Mar 30;291(5513):2608-13
• All: 151 reference(s)