Tg(CD2-Ahr*,CD2-GFP)AKno
Transgene Detail
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Symbol: |
Tg(CD2-Ahr*,CD2-GFP)AKno |
Name: |
transgene insertion A, Keiko Nohara |
MGI ID: |
MGI:3776513 |
Synonyms: |
Tg(hCD2-CA-AhR/GFP)A |
Transgene: |
Tg(CD2-Ahr*,CD2-GFP)AKno Location: unknown
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Alliance: |
Tg(CD2-Ahr*,CD2-GFP)AKno page
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Strain of Origin: |
C57BL/6J x DBA/2
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Transgene Type: |
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Transgenic (Constitutively active, Inserted expressed sequence, Reporter) |
Mutation: |
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Insertion
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Mutation details: This transgene was generated by coinjection of two transgene constructs, one expressing a constitutively active mutant mouse aryl hydrocarbon receptor and the other expressing green fluorescent protein, both under control of the T-cell lineage-specific human CD2 promoter. It was estimated by Southern blot analysis that Line A has two copies of the cointegrated transgenes. Transgene expression was detected by RT-PCR in thymus, spleen and lung and to a very minor extent in kidney. Flow cytometry analysis of GFP expression revealed a broader peak of fluorescence for thymocytes than for splenocytes; splenic Cd4+ and CD8+ T cells express GFP, while splenic B cells do not.
(J:97720)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:97720 Nohara K, et al., Constitutively active aryl hydrocarbon receptor expressed specifically in T-lineage cells causes thymus involution and suppresses the immunization-induced increase in splenocytes. J Immunol. 2005 Mar 1;174(5):2770-7 |
All: |
3 reference(s) |
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