Hif3a^tm1Mym
Targeted Allele Detail

Summary

• Symbol: Hif3a^tm1Mym
• Name: hypoxia inducible factor 3, alpha subunit; targeted mutation 1, Masayuki Yamamoto
• MGI ID: MGI:3776878
• Synonyms: NEPAS/HIF-3alpha^-
• Gene: Hif3a Location: Chr7:16765432-16796352 bp, - strand Genetic Position: Chr7, 9.15 cM, cytoband A2
• Alliance: Hif3a^tm1Mym page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132655
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of exon 2 was replaced with a GFP and floxed neo cassette. Expression of GFP was confirmed by immunohistochemistry. The absence of transcript expresison was confirmed by RT-PCR analysis on adult kidney extracts. This is a null allele. (J:132655)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hif3a Mutation: 38 strains or lines available

References

• Original: J:132655 Yamashita T, et al., Abnormal heart development and lung remodeling in mice lacking the hypoxia-inducible factor-related basic helix-loop-helix PAS protein NEPAS. Mol Cell Biol. 2008 Feb;28(4):1285-97
• All: 3 reference(s)