Ret^tm2(RET)Heno
Targeted Allele Detail

Summary

• Symbol: Ret^tm2(RET)Heno
• Name: ret proto-oncogene; targeted mutation 2, Hideki Enomoto
• MGI ID: MGI:3777555
• Synonyms: Ret^f(CFP), Ret^fCFP, Ret^flox
• Gene: Ret Location: Chr6:118128706-118174679 bp, - strand Genetic Position: Chr6, 55.86 cM, cytoband E3-F1
• Alliance: Ret^tm2(RET)Heno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132854
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A floxed human RET cDNA with an SV40 intron and poly-adenyation site followed by CFP with a poly-adenylation site and an frt-flanked neo cassette were inserted into exon 1 removing 39 base pairs including the start methionine codon and codons for the first 6 amino acids of the signal peptide. Germ-line, flp-mediated recombination was used to remove the neo cassette and leave a floxed RET and a CFP cassette. (J:132854)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ret Mutation: 54 strains or lines available

References

• Original: J:132854 Gould TW, et al., The neurotrophic effects of glial cell line-derived neurotrophic factor on spinal motoneurons are restricted to fusimotor subtypes. J Neurosci. 2008 Feb 27;28(9):2131-46
• All: 19 reference(s)