Nefl^{tm1(cre/ERT)Nat}
Targeted Allele Detail

Summary

• Symbol: Nefl^{tm1(cre/ERT)Nat}
• Name: neurofilament, light polypeptide; targeted mutation 1, Jeremy Nathans
• MGI ID: MGI:3777624
• Synonyms: Nefl^{tm1(cre/Esr1)Nat}, NFL-IRES-creER, NFL-IRES-creER(T)
• Gene: Nefl Location: Chr14:68321312-68326544 bp, + strand Genetic Position: Chr14, 35.46 cM, cytoband D3
• Alliance: Nefl^{tm1(cre/ERT)Nat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:144315
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Nefl^{tm1(cre/ERT)Nat} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nefl (MGI:97313)

• Mutation details: An IRES-creER cassette, a tetracycline resistance gene, and a Frt-flanked neomycin selection marker were all knocked in to the 3' UTR of the gene. The neomycin selection marker was removed by crossing to a C57BL/6 background Flp recombinase strain. Flp was then bred out of the line. When crossed with a Tg(CAG-Bgeo/ALPP)1Lbe reporter strain and given tamoxifen, cre expression was detected in ganglion cells and horizontal cells of the retina, and in a sparse number of neurons in the brain. (J:144315)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nefl (mouse) Summary of all recombinase alleles driven by Nefl. Comparative matrix view of recombinase activities Alliance mouse Nefl gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nefl Mutation: 17 strains or lines available

Notes

ES cell line = JHMI-ES1.

References

• Original: J:144315 Rotolo T, et al., Genetically-directed, cell type-specific sparse labeling for the analysis of neuronal morphology. PLoS One. 2008;3(12):e4099
• All: 5 reference(s)