Notch2^{Gt(AG0498)Wtsi}
Gene trapped Allele Detail

Summary

• Symbol: Notch2^{Gt(AG0498)Wtsi}
• Name: notch 2; gene trap AG0498, Wellcome Trust Sanger Institute
• MGI ID: MGI:3777790
• Synonyms: Notch2^AG0498, Notch2-betagal
• Gene: Notch2 Location: Chr3:97920854-98057683 bp, + strand Genetic Position: Chr3, 42.42 cM
• Alliance: Notch2^{Gt(AG0498)Wtsi} page

Mutation origin

• Mutant Cell Line: AG0498
• Germline Transmission: Earliest citation of germline transmission: J:132939
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd
• Project Collection: Sanger Inst. Gene Trap Res.

Mutation description

• Allele Type: Gene trapped (Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0lxr
• Mutation details: A gene trap vector with a strong splice acceptor and a beta-geo cassette inserted into the gene. This insertion created a fusion protein where the first 1,767 amino acids of the protein containing the extracellular domain, the transmembrane domain, and a small portion of the intracellular domain are fused to the reporter cassette. Reporter expression is detectable in the embryonic heart starting at E10.5. (J:132939)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  AG0498	CL459325 ; (MGI Seq Detail)	5' RACE	Chr3:98045807-98046997 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  16 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

10 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Notch2 Mutation: 99 strains or lines available

References

• Original: J:132939 Varadkar P, et al., Notch2 is required for the proliferation of cardiac neural crest-derived smooth muscle cells. Dev Dyn. 2008 Apr;237(4):1144-52
• All: 1 reference(s)