Gata4^tm1.1Sad
Targeted Allele Detail

Summary

• Symbol: Gata4^tm1.1Sad
• Name: GATA binding protein 4; targeted mutation 1.1, Stephen A Duncan
• MGI ID: MGI:3778685
• Synonyms: Gata4^Fx, Gata4^loxP
• Gene: Gata4 Location: Chr14:63436371-63509141 bp, - strand Genetic Position: Chr14, 33.24 cM
• Alliance: Gata4^tm1.1Sad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:92434
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to place a loxP-flanked thymidine kinase/neomycin phosphotransferase expression (tk/neo) cassette upstream of exon 3, and a third loxP site just downstream of exon 5 of the targeted gene. Correctly targeted ES cells were transiently transfected with a cre expressing plasmid to remove the selection cassette (generating the Gata4-loxP allele; with one loxP site upstream of exon 3 and one loxP site downstream of exon 5). (details provided by SA Duncan, personal communication.) (J:92434)

Disease models

Expression

In Mice Carrying this Mutation:	36 assay results 6 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gata4 Mutation: 36 strains or lines available

References

• Original: J:92434 Watt AJ, et al., GATA4 is essential for formation of the proepicardium and regulates cardiogenesis. Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12573-8
• All: 58 reference(s)