Lcp2^tm3Gak
Targeted Allele Detail

Summary

• Symbol: Lcp2^tm3Gak
• Name: lymphocyte cytosolic protein 2; targeted mutation 3, Gary A Koretzky
• MGI ID: MGI:3778759
• Synonyms: SLP-76 Y145F, SLP76^Y145F, Y145F
• Gene: Lcp2 Location: Chr11:33996928-34042281 bp, + strand Genetic Position: Chr11, 19.43 cM
• Alliance: Lcp2^tm3Gak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132995
• Parent Cell Line: iTL1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A tyrosine to phenylalanine substitution at position 145 (Y145F) was generated by a targeted A to T point mutation within exon 7 of the gene locus. For targeting purposes, a loxP and FRT flanked neomycin resistance cassette was placed downstream of exon 7. The selection cassette was subsequently removed by transient cre expression, leaving behind single loxP and FRT sites. The presence of the point mutation did not affect gene expression as determined by western blot analysis of thymocytes. (J:132995)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lcp2 Mutation: 41 strains or lines available

References

• Original: J:132995 Jordan MS, et al., Complementation in trans of altered thymocyte development in mice expressing mutant forms of the adaptor molecule SLP76. Immunity. 2008 Mar;28(3):359-69
• All: 10 reference(s)