Lcp2^tm4Gak
Targeted Allele Detail

Summary

• Symbol: Lcp2^tm4Gak
• Name: lymphocyte cytosolic protein 2; targeted mutation 4, Gary A Koretzky
• MGI ID: MGI:3778760
• Synonyms: SLP76^2F, SLP76^Y112-128F, Y112-128F, Y112/128F
• Gene: Lcp2 Location: Chr11:33996928-34042281 bp, + strand Genetic Position: Chr11, 19.43 cM
• Alliance: Lcp2^tm4Gak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132995
• Parent Cell Line: iTL1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Tyrosine to phenylalanine substitutions at positions 112 and 128 were generated by targeted A to T point mutations within exon 7 of the gene locus. For targeting purposes, a loxP and FRT flanked neomycin resistance cassette was placed downstream of exon 7. The selection cassette was subsequently removed by breeding mice with mice that express cre within the germline. The presence of the point mutations did not affect gene expression as determined by western blot analysis of thymocytes. (J:132995)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lcp2 Mutation: 41 strains or lines available

References

• Original: J:132995 Jordan MS, et al., Complementation in trans of altered thymocyte development in mice expressing mutant forms of the adaptor molecule SLP76. Immunity. 2008 Mar;28(3):359-69
• All: 8 reference(s)