Igh^tm1Jiac
Targeted Allele Detail

Summary

• Symbol: Igh^tm1Jiac
• Name: immunoglobulin heavy chain complex; targeted mutation 1, John Iacomini
• MGI ID: MGI:3778805
• Synonyms: M86VH
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:133032
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted
• Mutations: Deletion, Insertion
• Igh^tm1Jiac involves 1 genes/genome features (Igh) View all
• Mutation details: Hybridoma M86 produces a natural antibody that recognizes the carbohydrate antigen alphaGal. The recombined heavy chain from hybridoma M86 was used to replace the heavy chain cluster in ES cells. After germline transmission, the mice were crossed with a cre deleter strain to remove a neomycin selection cassette. Correct targeting was confirmed by genomic PCR. (J:133032)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 44 strains or lines available

References

• Original: J:133032 Benatuil L, et al., Ig Knock-In Mice Producing Anti-Carbohydrate Antibodies: Breakthrough of B Cells Producing Low Affinity Anti-Self Antibodies. J Immunol. 2008 Mar 15;180(6):3839-3848
• All: 1 reference(s)