Igk^tm1Jiac
Targeted Allele Detail

Summary

• Symbol: Igk^tm1Jiac
• Name: immunoglobulin kappa chain complex; targeted mutation 1, John Iacomini
• MGI ID: MGI:3778809
• Synonyms: M86VL
• Gene: Igk Location: Chr6:67532620-70703738 bp, + strand Genetic Position: Chr6, 30.89 cM, cytoband F1-pter

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:133032
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intergenic deletion
• Mutation details: Hybridoma M86 produces a natural antibody that recognizes the carbohydrate antigen alphaGal. The recombined kappa light chain from hybridoma M86 was used to replace the kappa light chain cluster in ES cells. After germline transmission, the mice were crossed with a cre deleter strain to remove a neomycin selection cassette. Correct targeting was confirmed by genomic PCR. (J:133032)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igk Mutation: 26 strains or lines available

References

• Original: J:133032 Benatuil L, et al., Ig Knock-In Mice Producing Anti-Carbohydrate Antibodies: Breakthrough of B Cells Producing Low Affinity Anti-Self Antibodies. J Immunol. 2008 Mar 15;180(6):3839-3848
• All: 1 reference(s)