Gt(ROSA)26Sor^{tm1(Tfrc*)Nca}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Tfrc*)Nca}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Nancy C Andrews
• MGI ID: MGI:3778905
• Synonyms: ROSA26^Tfr1R654A
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Tfrc*)Nca} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:133221
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Nucleotide substitutions
• Gt(ROSA)26Sor^{tm1(Tfrc*)Nca} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tfrc (MGI:98822)

• Mutation details: A Tfrc cDNA containing a point mutation that resulted in the amino acid substitution of alanine for arginine at position 654 (R654A) was knocked into the ROSA locus along with a floxed neo cassette. The neo cassette was subsequently removed by germ-line, cre-mediated recombination. This point mutation prevents binding to Tf but does not alter the interaction with Hfe. (J:133221)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:133221 Schmidt PJ, et al., The transferrin receptor modulates Hfe-dependent regulation of hepcidin expression. Cell Metab. 2008 Mar;7(3):205-14
• All: 2 reference(s)