Tpsb2^tm1.1Mfg
Targeted Allele Detail

Summary

• Symbol: Tpsb2^tm1.1Mfg
• Name: tryptase beta 2; targeted mutation 1.1, Michael F Gurish
• MGI ID: MGI:3783331
• Synonyms: Mcpt6^-, mMCP-6^-, Tpsb2^tm1Dle
• Gene: Tpsb2 Location: Chr17:25585282-25587070 bp, + strand Genetic Position: Chr17, 12.53 cM, cytoband A3.3
• Alliance: Tpsb2^tm1.1Mfg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:133373
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Parts of exon 2 and 3 were replaced with an EGFP expression cassette followed by two polyA stop sequences and a loxP-flanked neomycin resistance cassette. Founder mice were mated with cre transgenic mice to remove the neomycin selection cassette. Gene inactivation was confirmed by immunoblot analysis on bone marrow derived mast cells. Immunoblot analysis also confirmed that the adjacent Tpsab1 gene locus contained the intact 129 allele and not the null C57BL/6 allele. (J:133373)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tpsb2 Mutation: 12 strains or lines available

References

• Original: J:133373 Shin K, et al., Mouse Mast Cell Tryptase mMCP-6 Is a Critical Link between Adaptive and Innate Immunity in the Chronic Phase of Trichinella spiralis Infection. J Immunol. 2008 Apr 1;180(7):4885-4891
• All: 9 reference(s)