Tg(Cryaa-AKR1B1)222Smch
Transgene Detail

Summary

• Symbol: Tg(Cryaa-AKR1B1)222Smch
• Name: transgene insertion 222, Stephen SM Chung
• MGI ID: MGI:3783832
• Synonyms: CAR222
• Transgene: Tg(Cryaa-AKR1B1)222Smch Location: unknown
• Alliance: Tg(Cryaa-AKR1B1)222Smch page

Transgene origin

• Strain of Origin: (CBA x C57BL)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Cryaa-AKR1B1)222Smch expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  AKR1B1 (231)

  Akr1b1

  (MGI:1353494)

• Mutation details: The entire 1.4 Kb cDNA from a human AKR1B1 (hAR) clone with 36 bo 5' and 325 bp 3' untranslated regions was cloned into a vector containing the SV40 splice site and poly(A) sequence. The -341 to +49 region of the mouse Cryaa promoter was inserted at the 5' end of the AKR1B1 sequence and microninjected into fertilized oocytes. Northern blot analysis showed expression of AKR1B1 in the lens, but not in liver, brain, testis, or muscle. Five lines were characterized, CAR222, -223. -435, -440, and -648 with 30, 70, 100, 70, and 5 copies of the transgene. Lines 222 and 648 were used further and entered into the MGI database. (J:106642)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:106642 Lee AY, et al., Demonstration that polyol accumulation is responsible for diabetic cataract by the use of transgenic mice expressing the aldose reductase gene in the lens. Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2780-4
• All: 2 reference(s)