Erc2^tm1.1Sud
Targeted Allele Detail

Summary

• Symbol: Erc2^tm1.1Sud
• Name: ELKS/RAB6-interacting/CAST family member 2; targeted mutation 1.1, Thomas C Sudhof
• MGI ID: MGI:3783990
• Synonyms: E2X, ELKS2alpha^-
• Gene: Erc2 Location: Chr14:27344385-28200494 bp, + strand Genetic Position: Chr14, 16.51 cM
• Alliance: Erc2^tm1.1Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:133656
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A targeting vector was used to place a loxP site in the 5' UTR of the gene in the AflII restriction site 70 bp 5' of the ATG. A tetracysteine tag was inserted in frame into the first coding exon (from GGLSGSS to GGLSGCCPGCCSGSS) and an frt-neo-frt-loxp cassette was inserted 110 bp downstream of exon 1 into the genomic NdeI site. The line was crossed to cre-transgenic mice to excise the first coding exon. Total brain protein levels are normal. Rims1 is 30% more soluble in a detergent-free fractionation. (J:133656)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Erc2 Mutation: 112 strains or lines available

References

• Original: J:133656 Sudhof TC, Generation of an Erc2 mutant allele. MGI Direct Data Submission. 2008
• All: 3 reference(s)