Pkd1l3^tm1Sul
Targeted Allele Detail

Summary

• Symbol: Pkd1l3^tm1Sul
• Name: polycystic kidney disease 1 like 3; targeted mutation 1, Susan Sullivan
• MGI ID: MGI:3784517
• Synonyms: PKD1L3 KO
• Gene: Pkd1l3 Location: Chr8:110340828-110399305 bp, + strand Genetic Position: Chr8, 57.14 cM, cytoband A
• Alliance: Pkd1l3^tm1Sul page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:134024
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (C57BL/6J x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A 4.1 kb PacI-PacI genomic fragment of the gene including exons 17-21 was replaced with a 2.0 kb neomycin resistance cassette. The cassette was flanked by Frt and loxP sites and expression is driven by the pGKEM7 promoter: Frt-loxP-pGKEM7neo-Frt-loxP. This eliminates transmembrane domains 2 through 5 and causes a downstream shift in reading frame with consequent early stop codons. The predicted truncated protein lacks the ion channel pore and is therefore non functional. No expression was detected when the mice were examined by in situ hybridization of the taste buds. (J:134024, J:168530)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pkd1l3 Mutation: 154 strains or lines available

References

• Original: J:134024 Sullivan S, Generation of a Pkd1l3 knock-out mouse. MGI Direct Data Submission. 2008
• All: 6 reference(s)