Zfp148^Gt(XB878)Byg
Gene trapped Allele Detail

Summary

• Symbol: Zfp148^Gt(XB878)Byg
• Name: zinc finger protein 148; gene trap XB878, BayGenomics
• MGI ID: MGI:3784952
• Synonyms: ZBP-89^GT, Zfp148^gt
• Gene: Zfp148 Location: Chr16:33201145-33324273 bp, + strand Genetic Position: Chr16, 23.93 cM
• Alliance: Zfp148^Gt(XB878)Byg page

Mutation origin

• Mutant Cell Line: XB878
• Germline Transmission: Earliest citation of germline transmission: J:133931
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Hypomorph, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0pfs Vector Type: gene trap
• Mutation details: A gene trap vector containing a beta-geo cassette was inserted into intron 4 between exon 4 and exon 5. The insertion creates a 163 KDa fusion protein that contains the N-terminus and acidic domain to the beta-galactosidase and neo coding regions. The presence of the fusion protein was confirmed by western blot analysis. This allele is also a reporter allele. (J:133931)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XB878	CC156212 ; (MGI Seq Detail)	5' RACE	Chr16:33247611-33255343 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  104 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

4 assay results 1 RNA-Seq or microarray experiment(s)

Tumor Data

List all tumor models in MMHCdb carrying Zfp148^Gt(XB878)Byg

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfp148 Mutation: 112 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

Tetraploid complementation analysis of normal appearing embryos at E9.5 and E10.5 reveals grossly normal vasculature and closed neural tubes. Abundant, normal appearing erythrocytes are recovered from E10.5 complementation embryos indicating relatively normal primitive erythropoiesis occurs in absence of Zfp148. No live embryos are observed at E12.5-13.5.

References

• Original: J:133931 Woo AJ, et al., Identification of ZBP-89 as a novel GATA-1-associated transcription factor involved in megakaryocytic and erythroid development. Mol Cell Biol. 2008 Apr;28(8):2675-89
• All: 7 reference(s)