Rspo1^tm1.1Ktom
Targeted Allele Detail

Summary

• Symbol: Rspo1^tm1.1Ktom
• Name: R-spondin 1; targeted mutation 1.1, Kazuma Tomizuka
• MGI ID: MGI:3784965
• Gene: Rspo1 Location: Chr4:124880223-124902892 bp, + strand Genetic Position: Chr4, 57.96 cM
• Alliance: Rspo1^tm1.1Ktom page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:133934
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele was made using sequential targeting events. The first targeting event resulted in the insertion of a floxed neo cassette into exon 2 and the second event resulted in the replacement of exons 5 through 6 with a floxed puromycin resistance cassette. Transient cre expression was used to remove the selection cassettes and the remaining coding region of exon 2 and exon 3. The final allele lacks the entire coding region. The absence of transcript was confirmed by RT-PCR on ovary and testis extracts. (J:133934)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rspo1 Mutation: 21 strains or lines available

References

• Original: J:133934 Tomizuka K, et al., R-spondin1 plays an essential role in ovarian development through positively regulating Wnt-4 signaling. Hum Mol Genet. 2008 May 1;17(9):1278-91
• All: 1 reference(s)