Dot1l^{Gt(RRR032)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Dot1l^{Gt(RRR032)Byg}
• Name: DOT1 like histone lysine methyltransferase; gene trap RRR032, BayGenomics
• MGI ID: MGI:3784999
• Synonyms: Dot1L KO
• Gene: Dot1l Location: Chr10:80591040-80631295 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Dot1l^{Gt(RRR032)Byg} page

Mutation origin

• Mutant Cell Line: RRR032
• Germline Transmission: Earliest citation of germline transmission: J:133922
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• A gene trap vector was inserted into intron 12. (J:133922)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRR032	CG785000 ; (MGI Seq Detail)	5' RACE	Chr10:80609551-80618016 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  33 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dot1l Mutation: 72 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:133922 Steger DJ, et al., DOT1L/KMT4 recruitment and H3K79 methylation are ubiquitously coupled with gene transcription in mammalian cells. Mol Cell Biol. 2008 Apr;28(8):2825-39
• All: 4 reference(s)