Tg(Pmch-ATXN3*)3Rck
Transgene Detail

Summary

• Symbol: Tg(Pmch-ATXN3*)3Rck
• Name: transgene insertion 3, Jeffrey M Friedman
• MGI ID: MGI:3785576
• Synonyms: MCH-ataxin
• Transgene: Tg(Pmch-ATXN3*)3Rck Location: unknown
• Alliance: Tg(Pmch-ATXN3*)3Rck page

Transgene origin

• Strain of Origin: FVB/NJ

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Pmch-ATXN3*)3Rck expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ATXN3 (4287)

  Atxn3

  (MGI:1099442)

• Mutation details: A transgenic BAC vector contained a mouse pro-melanin-concentrating hormone (Pmch; MCH) promoterand the N terminus of the MCH gene controlling a human ataxin 3 (ATXN3) cDNA (encoding from amino acid 286 to the C terminus) containing 7 polyglutamine repeats that was introduced to the ATG site of the MCH BAC. The N terminus of the ATXN3 contained a hemagglutinin (HA) epitope and the C terminus contained a myelocytomatosis (MYC) oncogene epitope followed by the murine protamine 1 gene fragment that provided an intron and polyadenylation signal. The construct was injected into FVB/NJ fertilized oocytes. 5 transgenic lines were established. Line 1 was donated to JAX. Transgene expression in Pmch neurons was verified with double immunohistochemistry using MYC and MCH antibodies. (J:104264)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:104264 Alon T, et al., Late-onset leanness in mice with targeted ablation of melanin concentrating hormone neurons. J Neurosci. 2006 Jan 11;26(2):389-97
• All: 1 reference(s)