Gt(ROSA)26Sor^tm1Rkuhn
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm1Rkuhn
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Ralf Kuhn
• MGI ID: MGI:3785787
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm1Rkuhn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:133638
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (RMCE-ready)
• Mutation: Insertion
• Mutation details: This allele was created to facilitate insertion of vectors into the Rosa26 locus using recombinase-mediated cassette exchange. A splice acceptor followed by a pgk promoter driving an attP-flanked hygromycin selection gene was inserted into intron 1. Upon expression of C31 intergrase, the hyrgromycin cassette will be exchanged with a donor vector containing an attB-flanked cassette. (J:133638)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:133638 Hitz C, et al., Conditional brain-specific knockdown of MAPK using Cre/loxP regulated RNA interference. Nucleic Acids Res. 2007;35(12):e90
• All: 3 reference(s)