Gt(ROSA)26Sor^{tm4(RNAi:Gsk3a,Gsk3b)Rkuhn}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm4(RNAi:Gsk3a,Gsk3b)Rkuhn}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Ralf Kuhn
• MGI ID: MGI:3785819
• Synonyms: shGsk-3-dh^flox
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm4(RNAi:Gsk3a,Gsk3b)Rkuhn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:133340
• Parent Cell Line: IDG3.2 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Knockdown, RMCE-ready)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm4(RNAi:Gsk3a,Gsk3b)Rkuhn} involves 2 genes/genome features (Gsk3a, Gsk3b) View all
• Mutation details: Recombinase mediated cassette exchange (RMCE) was used to generate an allele where upon expression of Cre recombinase knockdown RNAis are generated to both Gsk3a and Gsk3b. Short hairpin RNAs were designed with a loxP-flanked stop cassette in the loop portion of the RNAi between the sense and anti-sense sequences. The RNAis were both put under the control of separate human U6 promoters and the entire cassette was placed downstream of a splice acceptor and a frt-flanked neomycin resistance gene. RMCE was then performed on ES cells containing the Gt(ROSA)26^tm1Rkuhn allele. Upon expression of cre recombinase, protein expression of Gsk3a and Gsk3b were reduced by 84% and 52%, respectively. (J:133340)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:133340 Steuber-Buchberger P, et al., Simultaneous Cre-mediated conditional knockdown of two genes in mice. Genesis. 2008 Mar;46(3):144-51
• All: 1 reference(s)