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Gt(ROSA)26Sortm4(RNAi:Gsk3a,Gsk3b)Rkuhn
Targeted Allele Detail
Summary
Symbol: Gt(ROSA)26Sortm4(RNAi:Gsk3a,Gsk3b)Rkuhn
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 4, Ralf Kuhn
MGI ID: MGI:3785819
Synonyms: shGsk-3-dhflox
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sortm4(RNAi:Gsk3a,Gsk3b)Rkuhn page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:133340
Parent Cell Line:  IDG3.2 (ES Cell)
Strain of Origin:  (C57BL/6J x 129S6/SvEvTac)F1
Mutation
description
Allele Type:    Targeted (Knockdown, RMCE-ready)
Mutation:    Insertion
  Gt(ROSA)26Sortm4(RNAi:Gsk3a,Gsk3b)Rkuhn involves 2 genes/genome features (Gsk3a, Gsk3b) View all
 
Mutation detailsRecombinase mediated cassette exchange (RMCE) was used to generate an allele where upon expression of Cre recombinase knockdown RNAis are generated to both Gsk3a and Gsk3b. Short hairpin RNAs were designed with a loxP-flanked stop cassette in the loop portion of the RNAi between the sense and anti-sense sequences. The RNAis were both put under the control of separate human U6 promoters and the entire cassette was placed downstream of a splice acceptor and a frt-flanked neomycin resistance gene. RMCE was then performed on ES cells containing the Gt(ROSA)26tm1Rkuhn allele. Upon expression of cre recombinase, protein expression of Gsk3a and Gsk3b were reduced by 84% and 52%, respectively. (J:133340)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  992 strains or lines available
References
Original:  J:133340 Steuber-Buchberger P, et al., Simultaneous Cre-mediated conditional knockdown of two genes in mice. Genesis. 2008 Mar;46(3):144-51
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory