En2^tm5.1Alj
Targeted Allele Detail

Summary

• Symbol: En2^tm5.1Alj
• Name: engrailed 2; targeted mutation 5.1, Alexandra L Joyner
• MGI ID: MGI:3789089
• Synonyms: En2-floxLacZ, En2^flox-taulacZ, En2^loxlz, En2-tau-lacZ, En2^tau-lacZ
• Gene: En2 Location: Chr5:28370692-28377164 bp, + strand Genetic Position: Chr5, 13.94 cM
• Alliance: En2^tm5.1Alj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:134516
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert a loxP-flanked tau-lacZ cassette followed by a neo cassette and then a third loxP site into the 5'-UTR of the targeted gene. Cre mediated recombination then removed the neo cassette leaving the floxed tau-lacZ cassette intact. (J:134516)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any En2 Mutation: 104 strains or lines available

References

• Original: J:95823 Sgaier SK, et al., Morphogenetic and cellular movements that shape the mouse cerebellum; insights from genetic fate mapping. Neuron. 2005 Jan 6;45(1):27-40
• All: 7 reference(s)