En1tm8.1Alj
Targeted Allele Detail
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Symbol: |
En1tm8.1Alj |
Name: |
engrailed 1; targeted mutation 8.1, Alexandra L Joyner |
MGI ID: |
MGI:3789091 |
Synonyms: |
En1flox |
Gene: |
En1 Location: Chr1:120530246-120535719 bp, + strand Genetic Position: Chr1, 52.74 cM, cytoband C2-qter
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Alliance: |
En1tm8.1Alj page
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Germline Transmission: |
Earliest citation of germline transmission:
J:134516
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Parent Cell Line: |
W4 (ES Cell)
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Strain of Origin: |
129S6/SvEvTac
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Allele Type: |
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Targeted (Conditional ready, No functional change) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A targeting construct was designed to insert an frt-flanked neomycin cassette and loxP site upstream, and a loxP site downstream of the second exon of the targeted gene. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with Black Swiss mice (to avoid rescue of the En1 mutant phenotype by the C57BL/6 background). The resulting En1flox-neo mice were then bred to 129 mice constitutively expressing Flp recombinase to remove the Frt-flanked neo cassette, generating En1flox offspring.
(J:134516)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:134516 Sgaier SK, et al., Genetic subdivision of the tectum and cerebellum into functionally related regions based on differential sensitivity to engrailed proteins. Development. 2007 Jun;134(12):2325-35 |
All: |
16 reference(s) |
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