Gt(ROSA)26Sor^{tm1.1(CAG-cre)Jphe}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-cre)Jphe}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Jean-Paul Herman
• MGI ID: MGI:3789474
• Synonyms: DiCre, Gt(ROSA)26Sor^{tm1.1(cre)Jphe}, Rosa26-DiCre
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-cre)Jphe} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:130959
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: rapamycin
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1.1(CAG-cre)Jphe} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details: The expression of a dimerizable cre was achieved by insertion of the cre60.F2 N-terminal cre moiety which is fused to FRB (ligand binding domain of FRAP/MTOR,an FKBP12-rapamycin associated protein) under the control of the endogenous promoter and a Caggs-cre59.F2 cassette, containing the C-teminal moiety of cre fused to FKBP12 (FK506-binding protein) under control of the CAG promoter, into the ROSA locus. A hygro cassette was also inserted into the locus for selection purposes. The split cre is inactive until dimerization is induced by administration of rapamycin. The hygro cassette was removed by crossing F1 Gt(ROSA)26Sor^tm1(cre)Jphe mice with FLPe mice. (J:130959)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:130959 Jullien N, et al., Conditional Transgenesis Using Dimerizable Cre (DiCre). PLoS One. 2007;2(12):e1355
• All: 1 reference(s)