Prkg1^tm1Meme
Targeted Allele Detail

Summary

• Symbol: Prkg1^tm1Meme
• Name: protein kinase, cGMP-dependent, type I; targeted mutation 1, Michael E Mendelsohn
• MGI ID: MGI:3789972
• Synonyms: LZM
• Gene: Prkg1 Location: Chr19:30541889-31742433 bp, - strand Genetic Position: Chr19, 25.17 cM, cytoband C2
• Alliance: Prkg1^tm1Meme page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:134634
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: Point mutations were introduced into codons 12, 19, 26, and 33 of exon 1 alpha, converting essential leucine zipper leucine/isoleucine residues into alanines. In addition a self-excising protamine-cre neo cassette was introduced into the intron after exon 1 alpha leaving behind a single loxp site. Immunoblot analysis of homozygous blood vessels detected expression of only the mutant protein. Pull down assay analysis confirmed the disruption of the interaction of the mutant protein with Mprip (J:134634)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkg1 Mutation: 60 strains or lines available

References

• Original: J:134634 Michael SK, et al., High blood pressure arising from a defect in vascular function. Proc Natl Acad Sci U S A. 2008 May 6;105(18):6702-7
• All: 4 reference(s)