Lgr4^{Gt(LST020)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Lgr4^{Gt(LST020)Byg}
• Name: leucine-rich repeat-containing G protein-coupled receptor 4; gene trap LST020, BayGenomics
• MGI ID: MGI:3790207
• Synonyms: Gpr48, Gpr48^-, Gpr68^-, Lgr4^m
• Gene: Lgr4 Location: Chr2:109747992-109844602 bp, + strand Genetic Position: Chr2, 56.65 cM
• Alliance: Lgr4^{Gt(LST020)Byg} page

Microphthalmia, iris hypoplasia, and iridiocorneal abnormalities in Lgr4^{Gt(LST020)Byg}/Lgr4^{Gt(LST020)Byg} mice

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: LST020
• Germline Transmission: Earliest citation of germline transmission: J:134299
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout)
• Mutation: Insertion of gene trap vector Vector: pGT2TMpfs Vector Type: secretory trap
• Mutation details: The gene trap vector pGT2TMpfs (contains a CD4 transmembrane domain, beta-geo cassette, IRES and PLAP reporter) was inserted into intron 1. The absence of full-length transcript was confirmed by RT-PCR on E12.5 embryo extracts. (J:134299)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  LST020	CC144589 ; (MGI Seq Detail)	5' RACE	Chr2:109748568-109748649 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  67 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Disease models

Expression

In Mice Carrying this Mutation:	49 assay results
In Structures Affected by this Mutation:	14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lgr4 Mutation: 94 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:134299 Weng J, et al., Deletion of G protein-coupled receptor 48 leads to ocular anterior segment dysgenesis (ASD) through down-regulation of Pitx2. Proc Natl Acad Sci U S A. 2008 Apr 22;105(16):6081-6
• All: 33 reference(s)