Sema3f^tm1Atai
Targeted Allele Detail

Summary

• Symbol: Sema3f^tm1Atai
• Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F; targeted mutation 1, Atsu Aiba
• MGI ID: MGI:3790328
• Synonyms: Sema3F(loxP)
• Gene: Sema3f Location: Chr9:107558699-107587674 bp, - strand Genetic Position: Chr9, 58.91 cM, cytoband F2
• Alliance: Sema3f^tm1Atai page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:134616
• Parent Cell Line: EB3 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A lacZ cassette was inserted into exon 3. Cre-mediated recombination removed the floxed selection cassette inserted downstream of exon 3. The absence of transcript expression was confirmed by northern blot and in situ hybridization. This was generated from Sema3f^tm2Atai. (J:134616)

Expression

In Mice Carrying this Mutation:

12 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sema3f Mutation: 41 strains or lines available

Notes

ES cells = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette.

References

• Original: J:134616 Ito K, et al., Semaphorin 3F confines ventral tangential migration of lateral olfactory tract neurons onto the telencephalon surface. J Neurosci. 2008 Apr 23;28(17):4414-22
• All: 4 reference(s)