Foxp3^{tm4(YFP/icre)Ayr}
Targeted Allele Detail

Summary

• Symbol: Foxp3^{tm4(YFP/icre)Ayr}
• Name: forkhead box P3; targeted mutation 4, Alexander Y Rudensky
• MGI ID: MGI:3790499
• Synonyms: Foxp3^Cre, Foxp3^{tm4(YFP/cre)Ayr}, Foxp3^YFP-Cre
• Gene: Foxp3 Location: ChrX:7445915-7461482 bp, + strand Genetic Position: ChrX, 3.41 cM
• Alliance: Foxp3^{tm4(YFP/icre)Ayr} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:134513
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Foxp3^{tm4(YFP/icre)Ayr} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Foxp3 (MGI:1891436)

• Mutation details: A YFP/icre fusion construct with an upstream IRES element and a downstream frt-flanked neomycin resistance cassette was placed into the downstream untranslated region of the gene. Chimeric mice were crossed with FLPe expressing mice to remove the neomycin selection cassette. YFP-cre expression based on YFP flouresecene was only detected in regulatory T cells. Crossing these mice with mice that contain a cre-mediated reporter gene also demonstrated low level cre expression in about 2-10% of hematopoietic lineage cells including immature and mature B cells, T cells, myeloid cells and bone marrow precursor cells. (J:134513)

Generation of the Cd28^tm1Ltu allele

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Foxp3 (mouse) Summary of all recombinase alleles driven by Foxp3. Comparative matrix view of recombinase activities Alliance mouse Foxp3 gene page

Disease models

Expression

In Mice Carrying this Mutation:

8 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Foxp3 Mutation: 58 strains or lines available

References

• Original: J:134513 Rubtsov YP, et al., Regulatory T cell-derived interleukin-10 limits inflammation at environmental interfaces. Immunity. 2008 Apr;28(4):546-58
• All: 372 reference(s)