Rr160^tm1Wtg
Targeted Allele Detail

Summary

• Symbol: Rr160^tm1Wtg
• Name: regulatory region 160; targeted mutation 1, William T Garrard
• MGI ID: MGI:3790771
• Synonyms: Ed^-, Igk^tm1Wtg
• Gene: Rr160 Location: Chr6:70720691-70721851 bp Genetic Position: Chr6, Syntenic
• Alliance: Rr160^tm1Wtg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:134885
• Parent Cell Line: SM1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: An enhancer element that lies far downstream of the C-kappa gene segment was targeted by homologous recombination. This "further downstream enhancer element" (Ed) is located 8 kb downstream from another downstream enhancer, the E3' enhancer element (Rr159). The 1.2kb Ed element was flanked by loxP sites with a floxed neomycin selection cassette also being placed immediately downstream. Founder mice were bred with cre transgenic mice to remove the Ed element and the selection cassette. Deletion of the Ed element was confirmed through Southern blotting. (J:134885)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr160 Mutation: 0 strains or lines available

References

• Original: J:134885 Xiang Y, et al., The Downstream Transcriptional Enhancer, Ed, positively regulates mouse Ig kappa gene expression and somatic hypermutation. J Immunol. 2008 May 15;180(10):6725-32
• All: 4 reference(s)