Fgf16^tm1(cre)Sms
Targeted Allele Detail

Summary

• Symbol: Fgf16^tm1(cre)Sms
• Name: fibroblast growth factor 16; targeted mutation 1, Suzanne L Mansour
• MGI ID: MGI:3790778
• Synonyms: Fgf16^IRESCreNeo
• Gene: Fgf16 Location: ChrX:104807885-104818545 bp, + strand Genetic Position: ChrX, 47.2 cM, cytoband C3
• Alliance: Fgf16^tm1(cre)Sms page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:139718
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Fgf16^tm1(cre)Sms expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Fgf16 (MGI:1931627)

• Mutation details: The Cre recombinase coding sequence, preceded by an internal ribosomal entry site (IRES) and followed by a frt-flanked neomycin resistance cassette, interrupts exon 1 so as to disrupt codon 63. RT-PCR using primers to sequences upstream of the insertion site and in exon 2 detects the expected Fgf16 transcripts in E9.0 wild-type and heterozygous female embryos, but none in hemizygous male embryos. Cre recombinase is not expressed from this allele. Crossing mice bearing this allele with mice expressing FLPe recombinase yields progeny in which the neo cassette has been excised and Cre recombinase is expressed under control of the endogenous Fgf16 promoter. (J:139718)
• Inheritance: Recessive

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Fgf16 (mouse) Summary of all recombinase alleles driven by Fgf16. Alliance mouse Fgf16 gene page

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fgf16 Mutation: 7 strains or lines available

References

• Original: J:139718 Hatch EP, et al., Fgf16(IRESCre) mice: a tool to inactivate genes expressed in inner ear cristae and spiral prominence epithelium. Dev Dyn. 2009 Feb;238(2):358-66
• All: 2 reference(s)