Igh^tm12Cgn
Targeted Allele Detail

Summary

• Symbol: Igh^tm12Cgn
• Name: immunoglobulin heavy chain complex; targeted mutation 12, University of Cologne
• MGI ID: MGI:3790800
• Synonyms: pII^inv
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66031
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Intergenic deletion
• Mutation details: Through homologous recombination a modified version of the vector used to create Igh^tm2Cgn replaced the endogenous sequence. This vector replaces a 3' Igh-D element (DQ52) and Igh-J elements with a recombined antibody variable region derived from a 4-hydroxy-3-nitrophenyl acetyl binding antibody (B1-8). The variable region, a leader sequence that includes a termination codon, and the endogenous IgH promoter were placed in an inverted fashion so that the IgH promoter was adjacent to the intronic enhancer that lies upstream of the heavy chain constant gene segments. (J:66031)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 44 strains or lines available

References

• Original: J:66031 Bross L, et al., DNA double-strand breaks in immunoglobulin genes undergoing somatic hypermutation. Immunity. 2000 Nov;13(5):589-97
• All: 3 reference(s)