E2f6^tm1Lees
Targeted Allele Detail

Summary

• Symbol: E2f6^tm1Lees
• Name: E2F transcription factor 6; targeted mutation 1, Jacqueline A Lees
• MGI ID: MGI:3790807
• Gene: E2f6 Location: Chr12:16860932-16876753 bp, + strand Genetic Position: Chr12, 8.04 cM
• Alliance: E2f6^tm1Lees page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:134271
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 4 through 8, encoding the DNA binding, leucine zipper, and marked box domains were replaced via homologous recombination with a floxed PGK-neo cassette that includes a stop codon at the beginning. Western blot analysis of homozygous MEFs confirmed the absence of protein expression. (J:134271)

Expression

In Mice Carrying this Mutation:	27 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any E2f6 Mutation: 15 strains or lines available

References

• Original: J:134271 Courel M, et al., E2f6 and Bmi1 cooperate in axial skeletal development. Dev Dyn. 2008 May;237(5):1232-42
• All: 2 reference(s)