Tg(CAG-EGFP/CETN2)3-4Jgg
Transgene Detail

Summary

• Symbol: Tg(CAG-EGFP/CETN2)3-4Jgg
• Name: transgene insertion 3-4, Joseph G Gleeson
• MGI ID: MGI:3793421
• Synonyms: Centrin 2-GFP, GFP-CETN2
• Transgene: Tg(CAG-EGFP/CETN2)3-4Jgg Location: unknown
• Alliance: Tg(CAG-EGFP/CETN2)3-4Jgg page

Transgene origin

• Strain of Origin: BALB/c x C57BL/6

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The GFP-CETN2 transgene was designed with a chicken beta-actin promoter (with cytomegalovirus immediate early enhancer and beta-actin intron) upstream of an EGFP-centrin 2 fusion protein (containing the full length human centrin 2 cDNA cloned in-frame into the C-terminal of enhanced green fluorescent protein), and followed by a beta-globin polyA sequence. Two lines were maintained (18-1 and 3-4) and line 3-4 was donated to The Jackson Laboratory. These lines show consistent, robust expression of GFP in centrosomes (particularly in cerebellar granule cells). (J:91267)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:91267 Higginbotham H, et al., Transgenic mouse line with green-fluorescent protein-labeled Centrin 2 allows visualization of the centrosome in living cells. Transgenic Res. 2004 Apr;13(2):155-64
• All: 42 reference(s)