Gata6^tm3.1Sad
Targeted Allele Detail

Summary

• Symbol: Gata6^tm3.1Sad
• Name: GATA binding protein 6; targeted mutation 3.1, Stephen A Duncan
• MGI ID: MGI:3793425
• Synonyms: Gata4^-Gata6^-
• Gene: Gata6 Location: Chr18:11052510-11085636 bp, + strand Genetic Position: Chr18, 5.63 cM
• Alliance: Gata6^tm3.1Sad page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:135185
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 3 and 4 were floxed and cre-mediated recombination was used to remove the exons as well as the selection cassette. Gene targeting was performed ES cells that were homozygous for Gata4^tm1.2Sad. The absence of Gata 4 and 6 transcripts was confirmed by RT-PCR on embryoid body extracts. (J:135185)

Expression

In Mice Carrying this Mutation:

23 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gata6 Mutation: 35 strains or lines available

References

• Original: J:135185 Zhao R, et al., Loss of both GATA4 and GATA6 blocks cardiac myocyte differentiation and results in acardia in mice. Dev Biol. 2008 May 15;317(2):614-9
• All: 3 reference(s)