Tg(H2-Ea)16Dim
Transgene Detail

Summary

• Symbol: Tg(H2-Ea)16Dim
• Name: transgene insertion 16, Diane Mathis
• MGI ID: MGI:3793709
• Synonyms: Ea16, Tg(Ea16)1Strasbourg
• Transgene: Tg(H2-Ea)16Dim Location: unknown
• Alliance: Tg(H2-Ea)16Dim page

Transgene origin

• Strain of Origin: Not Applicable

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(H2-Ea)16Dim expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	H2-I (MGI:95903)

• Mutation details: An 8.2 kb construct containing all the mouse (A/J) H2-Ea kappa exons (from clone lambda Charon 4) plus 2 kb of DNA 5' to the cap site and 1.4 kb 3' to the poly(A) addition site was microinjected into (C57BL/6 x SJL) embryos. Two lines 23 and 16 were generated with line 16 carrying 12 copies of the transgene in its genome and line 23 having 4 copies. Northern blots and S1 nuclease analysis showed transcripts in the spleen. Line 16 produced more transcripts than line 4, and both lines showed correct initiation sites of transcription. (J:111128)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Transgenic lines were derived from two transgenic founder mice and designated line 16 and line 23. Line 16 exhibits slightly higher antibody titers and mRNA levels in response to synthetic antigen.

References

• Original: J:111128 Le Meur M, et al., Correcting an immune-response deficiency by creating E alpha gene transgenic mice. Nature. 1985 Jul 4-10;316(6023):38-42
• All: 6 reference(s)