Tg(SLC18A3-cre)Misa
Transgene Detail

Summary

• Symbol: Tg(SLC18A3-cre)Misa
• Name: transgene insertion, Hidemi Misawa
• MGI ID: MGI:3793863
• Synonyms: VAChT-cre
• Transgene: Tg(SLC18A3-cre)Misa Location: unknown
• Alliance: Tg(SLC18A3-cre)Misa page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(SLC18A3-cre)Misa expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  SLC18A3 (6572)

  Slc18a3

  (MGI:1101061)

• Mutation details: The transgenic construct consisted of an 11.3 kb fragment of the human cholinergic gene locus, containing the first 5 exons of CHAT and the first coding exon of SLC18A3 (located in intron 1 of CHAT). A cassette containing cre followed by IRES-EGFP was inserted at the start codon of the SLC18A3 gene. EGFP expression was undetected. The 11.3 kb fragment included various promoter elements including a distal enhancer, RE1/NRSE, and NGFRE. Founder mice contained approximately 50 copies. Expression of cre recombinase by the human SLC18A3 promoter began at postnatal day 7 and was restricted to a subset of cholinergic neurons in the somatomotor nuclei and medial habenular nucleus. Maximal levels of expression were reached around 8 months ofage, later onset than in the Tg(SLC18a3-cre)KMisa line. Transgenic expression was not observed in visceromotor or other central or peripheral cholinergic neurons. (J:86160)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	SLC18A3 (human) Summary of all recombinase alleles driven by SLC18A3. Alliance human SLC18A3 gene page

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:135268 Yamanaka K, et al., Astrocytes as determinants of disease progression in inherited amyotrophic lateral sclerosis. Nat Neurosci. 2008 Mar;11(3):251-3
• All: 7 reference(s)