Pparg^tm1Laz
Targeted Allele Detail

Summary

• Symbol: Pparg^tm1Laz
• Name: peroxisome proliferator activated receptor gamma; targeted mutation 1, Mitchell A Lazar
• MGI ID: MGI:3794857
• Synonyms: PPARgamma-S112A
• Gene: Pparg Location: Chr6:115337912-115467360 bp, + strand Genetic Position: Chr6, 53.41 cM, cytoband E3-F1
• Alliance: Pparg^tm1Laz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:135904
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 2 was replaced with an exon 2 containing a nucleotide substitution that resulted in the amino acid substitution of alanine for serine at position 112 (S112A). A floxed neo cassette downstream of the modified exon 2 used for selection purposes was removed by cre-mediated recombination in ES cells. This mutant version of PPARgamma is nonphosphorylatable and has more biological activity. (J:135904)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pparg Mutation: 41 strains or lines available

References

• Original: J:135904 Rangwala SM, et al., Genetic modulation of PPARgamma phosphorylation regulates insulin sensitivity. Dev Cell. 2003 Oct;5(4):657-63
• All: 4 reference(s)