Rela^tm1.2Gho
Targeted Allele Detail

Summary

• Symbol: Rela^tm1.2Gho
• Name: v-rel reticuloendotheliosis viral oncogene homolog A (avian); targeted mutation 1.2, Sankar Ghosh
• MGI ID: MGI:3794988
• Synonyms: p65-R274A, S276A(RAPA)
• Gene: Rela Location: Chr19:5687511-5698158 bp, + strand Genetic Position: Chr19, 4.34 cM, cytoband B1-3
• Alliance: Rela^tm1.2Gho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:134684
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: By homologous recombination in ES cells, endogenous DNA sequence for arginine 274 and serine 276 residues in exon 7 of Rela gene were replaced with codons for alanine at both locations followed by a neo cassette flanked by two loxP sites inserted into intron after Exon 7. A neo cassette was then deleted either in the recombined ES cells by transfection of cre-recombinase or in mice by crossing with splicer mice harboring a Cre transgene, leaving only single loxP sites. The presence of the mutation was confirmed by RT-PCR and DNA sequencing. Somewhat reduced amount mutant protein was translated. (J:134684)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rela Mutation: 28 strains or lines available

References

• Original: J:134684 Dong J, et al., Repression of gene expression by unphosphorylated NF-kappaB p65 through epigenetic mechanisms. Genes Dev. 2008 May 1;22(9):1159-73
• All: 1 reference(s)