Pdpk1^tm1Dral
Targeted Allele Detail

Summary

• Symbol: Pdpk1^tm1Dral
• Name: 3-phosphoinositide dependent protein kinase 1; targeted mutation 1, Dario R Alessi
• MGI ID: MGI:3795190
• Synonyms: PDK1^K465E
• Gene: Pdpk1 Location: Chr17:24292654-24369898 bp, - strand Genetic Position: Chr17, 12.23 cM, cytoband A3.3
• Alliance: Pdpk1^tm1Dral page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:136089
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 12 wass replaced with a floxed neo cassette and an exon 12 containing a nucleotide substitution that results in the amino acid substitution of glutamic acid for lysine at position 465 (K465E). The neo cassette was removed by germ line, cre-mediated recombination. (J:136089)

Expression

In Mice Carrying this Mutation:	42 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Pdpk1^tm1Dral

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdpk1 Mutation: 138 strains or lines available

References

• Original: J:136089 Bayascas JR, et al., Mutation of the PDK1 PH domain inhibits protein kinase B/Akt, leading to small size and insulin resistance. Mol Cell Biol. 2008 May;28(10):3258-72
• All: 8 reference(s)