Mael^tm1Bort
Targeted Allele Detail

Summary

• Symbol: Mael^tm1Bort
• Name: maelstrom spermatogenic transposon silencer; targeted mutation 1, Alex Bortvin
• MGI ID: MGI:3795667
• Gene: Mael Location: Chr1:166028954-166066313 bp, - strand Genetic Position: Chr1, 73.56 cM
• Alliance: Mael^tm1Bort page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:140402
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 5.9 kb of genomic DNA encompassing the CpG island and first five exons of the gene were replaced with a loxP-flanked hygromycin-thymidine kinase selection cassette. Transient expression of cre recombinase in correctly targeted ES cells excised the selection cassette, leaving behind a single loxP site. Gene inactivation was confirmed by immunoblotting of testicular lysates. (J:140402)
• Inheritance: Recessive

Expression

In Mice Carrying this Mutation:	3 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mael Mutation: 25 strains or lines available

References

• Original: J:140402 Soper SF, et al., Mouse maelstrom, a component of nuage, is essential for spermatogenesis and transposon repression in meiosis. Dev Cell. 2008 Aug;15(2):285-97
• All: 4 reference(s)