Enam^tm1.1Jcch
Targeted Allele Detail

Summary

• Symbol: Enam^tm1.1Jcch
• Name: enamelin; targeted mutation 1.1, Jan C C Hu
• MGI ID: MGI:3796583
• Synonyms: Enam^lacZ
• Gene: Enam Location: Chr5:88635834-88653908 bp, + strand Genetic Position: Chr5, 43.66 cM, cytoband E2
• Alliance: Enam^tm1.1Jcch page

Tooth abnormalities in Enam^tm1.1Jcch/Enam⁺ and Enam^tm1.1Jcch/Enam^tm1.1Jcch mice at 7 weeks of age

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:136524
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of exon 3 through exon 7 was replaced with a lacZ and floxed neo cassette. The neo cassette was subsequently removed by crossing targeted mice with transgenic mice expressing cre recombinase in the germline. The absence of protein product was confirmed by western blot analysis on enamel extracts. This is a reporter allele and a null allele. (J:136524)

Disease models

Expression

In Structures Affected by this Mutation:

12 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Enam Mutation: 71 strains or lines available

References

• Original: J:136524 Hu JC, et al., Enamel defects and ameloblast-specific expression in Enam knock-out/lacz knock-in mice. J Biol Chem. 2008 Apr 18;283(16):10858-71
• All: 7 reference(s)