Tg(Igh-3VD93)343Ust
Transgene Detail

Summary

• Symbol: Tg(Igh-3VD93)343Ust
• Name: transgene insertion 343, Ursula Storb
• MGI ID: MGI:3798613
• Synonyms: 343-1, g2b, Tg(Igh)Bri49
• Transgene: Tg(Igh-3VD93)343Ust Location: unknown
• Alliance: Tg(Igh-3VD93)343Ust page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Igh-3VD93)343Ust expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Igh (MGI:96442)

• Mutation details: A 6.6 fragmnent containing the C region of the mouse gamma 2b (Igh-3) gene from clone VD93 (which shows antibody activity to Pseudomonas aeruginosa) and membrane genomic sequences from clone pgamma2b was subcloned into the pUC13 vector (pCgamma2b-mb). The 4.5 kb fragment of containing the V_H region of clone VD93 was cloned into pCgamma2b-mb. A clone with proper orientation of V_pseu-Cgamma2b-mb (clone pVCM) was identified. An 11.2 kb fragment from pVCM containing the V_pseu-Cgamma2b-mb region and 150 bp of vector sequence was used to produce transgenic mice of a (C57BL/6 x SJL)F2 background. Several lines were generated with 2 lines, 343-1 and 348-4, being studied in detail. These lines have 16 and 150 copies of the transgene respectively. (J:135210)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Transgenic lines were derived from two transgenic founder mice and designated line 343-1 and line 348A (copy numbers 16 and 150 respectively). Both lines exhibit a decrease in pre-B cell numbers, however, line 343-1 was characterized in more detail.

References

• Original: J:135210 Tsang H, et al., Cloning of a gamma 2b gene encoding anti-Pseudomonas aeruginosa H chains and its introduction into the germ line of mice. J Immunol. 1988 Jul 1;141(1):308-14
• All: 2 reference(s)