Ctrq3^129S2/SvPas
QTL Variant Detail

Summary

• QTL variant: Ctrq3^129S2/SvPas
• Name: C. trachomatis resistance QTL 3; 129S2/SvPas
• MGI ID: MGI:3798695
• QTL: Ctrq3 Location: Chr11:44630038-76854757 bp Genetic Position: Chr11, cM position of peak correlated region/allele: 35.4 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: 129S2/SvPas

Variant description

• Allele Type: QTL
• Inheritance: Not Specified

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:106111

Linkage analysis was performed on 169 animals from a (C57BL/6J x C3H/HeJ)F2 intercross to identify loci associated with susceptibility to Chlamydia trachomatis infection. Genome scan was conducted using 93 microsatellite markers at an average spacing of 16 cM. Parental strain C57BL/6J is more resistant to infection with C. trachomatis compared to parental strain C3H/HeJ. Animals were phenotyped by analyzing bacterial load in spleens after infection with C. trachomatis. In vitro analysis was also performed using INF-gamma-treated mouse embryonic fibroblasts (MEFs) derived from (C57BL/6J x C3H/HeJ)F2 animals infected with C. trachomatis.

Ctrq1 (C. trachomatis resistance QTL 1) mapped to 69 cM on mouse Chromosome 2 near D2Mit277 (LRS=20.5). C3H/HeJ-derivedalleles at Ctrq1 confer susceptibility to C. trachomatis infection with a recessive mode of inheritance.

Ctrq2 (C. trachomatis resistance QTL 2) mapped to 55 cM on mouse Chromosome 3 near D3Mit57 (LRS=19.9). The 1.5 LOD support interval spans 44.8 cM to66.2 cM from D3Mit49 to D3Mit350. C3H/HeJ-derived alleles at Ctrq2 confer resistance to C. trachomatis infection with dominant inheritance.

Ctrq3 (C. trachomatis resistance QTL 3) mapped to 32 cM on mouse Chromosome 11 near D11Mit143 (LRS=19.5). C3H/HeJ-derived alleles at Ctrq3 confer susceptibility to C. trachomatis infection with dominant inheritance. This locus was reaffirmed by constructing a congenic line carrying the entire Ctrq3 region (D11Mit20 to D11Mit245) derived from susceptible strain C3H/HeJ on the genetic background of resistant strain C57BL/6J. B6.C3H-Ctrq3 congenics exhibit significantly greater bacterial load after C. trachomatis infection compared to C57BL/6J controls. IFN-gamma-treated MEFs derived from heterozygous B6.C3H-Ctrq3 animals infected with C. trachomatis also exhibit a greater level of bacterial replication compared to controls.

J:113755

Ctrq3 (C. trachomatis resistance 3) was previously mapped to 32 cM on mouse Chromosome 11 in a (C57BL/6J x C3H/HeJ)F2 intercross. Parental strain C57BL/6J is resistant to C. trachomatis infection compared to parental strain C3H/HeJ. Congenic line analysis refined the Ctrq3 interval to a 1.2 Mb region between D11Mit164 and D11Zbh12. This region contains 18 genes, two of which are proposed as candidates for Ctrq3. Igtp (32 cM) and Irgb10 both exhibit amino acid polymorphisms between C57BL/6J and C3H/HeJ. In vitro analysis of Irgb10 expression showed 20-fold increased expression in IFN-gamma treated mouse embryonic fibroblasts (MEFs) derived from C57BL/6J compared to C3H/HeJ. In addition, MEFs derived from heterozygous Ctrq3 congenics showed 2-fold decreased Irgb10 expression compared to background strain C57BL/6J.

References

• Original: J:134525 Coers J, et al., Chlamydia muridarum evades growth restriction by the IFN-gamma-inducible host resistance factor Irgb10. J Immunol. 2008 May 1;180(9):6237-45
• All: 1 reference(s)