Dgcr8^Gt(XH157)Byg
Gene trapped Allele Detail

Summary

• Symbol: Dgcr8^Gt(XH157)Byg
• Name: DGCR8, microprocessor complex subunit; gene trap XH157, BayGenomics
• MGI ID: MGI:3798965
• Synonyms: Dgcr8^-
• Gene: Dgcr8 Location: Chr16:18071812-18107110 bp, - strand Genetic Position: Chr16, 11.31 cM, cytoband B1
• Alliance: Dgcr8^Gt(XH157)Byg page

Mutation origin

• Mutant Cell Line: XH157
• Germline Transmission: Earliest citation of germline transmission: J:136969
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The beta-geo gene trap was inserted into intron 8. The fusion transcript and protein product were confirmed by northern blot and western blot, respectively, in heterozygous mice. Wild-type protein and transcript levels are reduced in heterozygous mice. (J:136969)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XH157	CC199529 ; (MGI Seq Detail)	5' RACE	Chr16:18095030-18096271 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  64 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dgcr8 Mutation: 67 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:136969 Stark KL, et al., Altered brain microRNA biogenesis contributes to phenotypic deficits in a 22q11-deletion mouse model. Nat Genet. 2008 Jun;40(6):751-60
• All: 11 reference(s)