Wap^tm2(rtTA)Kuw
Targeted Allele Detail

Summary

• Symbol: Wap^tm2(rtTA)Kuw
• Name: whey acidic protein; targeted mutation 2, Kay Uwe Wagner
• MGI ID: MGI:3799057
• Gene: Wap Location: Chr11:6585482-6588637 bp, - strand Genetic Position: Chr11, 4.61 cM
• Alliance: Wap^tm2(rtTA)Kuw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:148684
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

Allele Type: Targeted (Transactivator) Mutation: Insertion Mutation details: The coding sequence for rtTA was inserted into the 5'-UTR along with a floxed neomycin selection cassette. The neo selection cassette was eliminated in founder line 7A3 by crossing properly targeted mice to Tg(MMTV-cre)1Mam transgenic animals. When mice were given doxycycline, transgene expression was restricted to mammary epithelial cells as determined by crosses with two different rtTA reporter strains. (J:148684)

Generation of the Wap^tm2(rtTA)Kuw allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Wap Mutation: 33 strains or lines available

References

• Original: J:148684 Creamer BA, et al., Longitudinal analysis of mammogenesis using a novel tetracycline-inducible mouse model and in vivo imaging. Genesis. 2009 Apr;47(4):234-45
• All: 3 reference(s)