Syt5^tm1Sud
Targeted Allele Detail

Summary

• Symbol: Syt5^tm1Sud
• Name: synaptotagmin V; targeted mutation 1, Thomas C Sudhof
• MGI ID: MGI:3799073
• Gene: Syt5 Location: Chr7:4542764-4550540 bp, - strand Genetic Position: Chr7, 2.62 cM, cytoband A1
• Alliance: Syt5^tm1Sud page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:137340
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: he Syt5 knock-in was created by inserting a tetracysteine motif tag dimer (Flag-CCPGCC-Flag-Flag-CCPGCC-Flag) in exon 3 of the gene. Exon 3 was additionally flanked by loxP sites and an Frt-flanked neomycin resistance cassette was placed 3' of the exon. The neomycin fragment may have been removed. (J:137340)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Syt5 Mutation: 15 strains or lines available

References

• Original: J:137340 Sudhof TC, Generation of a series of synaptotagmin mutant alleles. MGI Direct Data Submission. 2008
• All: 3 reference(s)