Hsd17b1^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Hsd17b1^{tm1(KOMP)Vlcg}
• Name: hydroxysteroid (17-beta) dehydrogenase 1; targeted mutation 1, Velocigene
• MGI ID: MGI:3799948
• Synonyms: Hsd17b1^tm1Vlcg
• Gene: Hsd17b1 Location: Chr11:100969237-100971353 bp, + strand Genetic Position: Chr11, 64.17 cM
• Alliance: Hsd17b1^{tm1(KOMP)Vlcg} page
• IMPC: Hsd17b1 gene page

Mutation origin

• Mutant Cell Lines: 10231B-A2, 10231B-F1, 10231B-G7, 10231B-H6, 10231B-H9, 10231B-H11
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 1829bp between positions 100939740-100941568 of Chromosome 11 (Genome Build37). The conding sequence is completely replaced with a lacZ/neomycin resistance cassette. RT-PCR confirmed the absence of transcript expression in ovaries. (J:136110, J:225533)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Hsd17b1 Mutation: 14 strains or lines available

Notes

Cell line 10231B-H11 was successfully used to make chimeric mice. Germline transmission was/was not accomplished. J:225533

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 4 reference(s)